empty vectors oe-nc (Shanghai GenePharma)
90
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Shanghai GenePharma
empty vectors oe-nc
Empty Vectors Oe Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector+(nc-oe/empty+vector/pm39843621-55-6-28
Average 90 stars, based on 1 article reviews
Empty Vectors Oe Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector+(nc-oe/empty+vector/pm39843621-55-6-28
Average 90 stars, based on 1 article reviews
empty vectors oe-nc - by Bioz Stars,
2026-09
90/100 stars
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Control:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Over Expression:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Plasmid Preparation:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. shRNA:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Negative Control:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Synthesized:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Cell Culture:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Concentration Assay:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Construct:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Small Interfering RNA:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Transfection:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Quantitative RT-PCR:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Expressing:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Western Blot:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Cell Counting:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Enzyme-linked Immunosorbent Assay:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. Real-time Polymerase Chain Reaction:Article Title: FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway Article Snippet: DUSP12 plasmid (Oe-DUSP12), FOXP1 plasmid (Oe-FOXP1) and the empty vector plasmid (Oe-NC), small interfering (si)RNAs targeting FOXP1 (si-FOXP1#1 or si-FOXP1#2) and the scrambled siRNA negative control (si-NC) were synthesized by Article Title: Serum long non-coding Ribonucleic Acid H19 serves as a biomarker for systemic lupus erythematosus and participates in the disease progression. Article Snippet: Aim: This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).. Methods: The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR).. Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Article Title: YAP1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF-κB/JMJD3 pathway Article Snippet: The YAP1 overexpression vector pcDNA-TRIM31 (Oe-YAP1) and empty control vector pcDNA (Oe-NC) were constructed by Shanghai GenePharma Co., Ltd. Small interfering RNA (siRNA) against YAP1 (si-YAP1) and siRNA negative control (si-NC) were synthesized by Article Title: GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Article Snippet: Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis.. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism.. The expression of GABRP was measured using the quantitative real-time PCR and western blot. Article Title: Role of TRIM22 in ulcerative colitis and its underlying mechanisms Article Snippet: The pGPH1 vector carrying short hairpin (sh)RNA plasmids (sh-TRIM30-1; 5′-CAGTATAGAAGTTACAATA-3′ and sh-TRIM30-2; 5′-GGTGAATATCTGTGCACAA-3′), a sh-TRIM22 plasmid (5′-GGAAGATGACATCAGACAA-3′), a sh-KLF2-1 plasmid (5′-GCACCGACGACGACCTCAA-3′) and sh-KLF2-2 plasmid (5′-GAAGCGCGGCCGCCGCTCTTG-3′), a negative control (NC) shRNA plasmid (sh-NC), pCDNA3.1 vector targeting KLF2 (Oe-KLF2) and an empty NC vector (Oe-NC) were all purchased from Shanghai GenePharma Co., Ltd. and were dissolved in sterile phosphate-buffered saline (PBS; Beyotime Institute of Biotechnology). Article Title: Leucine rich alpha-2-glycoprotein 1 (Lrg1) silencing protects against sepsis-mediated brain injury by inhibiting transforming growth factor beta1 (TGFβ1)/SMAD signaling pathway Article Snippet: Small interfering (si) RNAs targeting Lrg1 (si-Lrg1-1 and si-Lrg1-2) and the corresponding negative control (si-NC), TGFβ1 overexpression plasmid (Oe-TGFβ1) and the empty vector plasmid (Oe-NC) were obtained from Shanghai GenePharma Co., Ltd (Shanghai, China) [ ]. Article Title: Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Article Snippet: Intracerebral hemorrhage (ICH) induces severe neurological damage, and its progression is driven by METTL3.. This study aimed to investigate the role of METTL3 in ICH via in vitro experiments.. For this purpose, HT-22 cells were treated with hemin to mimic ICH in vitro, followed by evaluating cell pyroptosis using flow cytometry, lactic dehydrogenase release analysis, enzyme-linked immunosorbent assay, and western blotting. |